Blood stains have a lot of lipids (fats), iron particles, and other substances that help them attach to other things very well, especially natural fabric fibers like cotton). Removing it is challenging. It is more challenging when dried, and considerably more challenging when heatedly dried and set.
Protein Content
Hemoglobin and other proteins found in the blood may attach strongly to fiber cloths. Proteins are difficult to common cleaning techniques because of their tendency to coagulate and form solid bonds.
Hemoglobin Oxidation
The iron in hemoglobin oxidizes when blood comes in contact with oxygen, causing a chemical shift that might make the blood stain more permanent and challenging to remove.
The blood stain may get permanently stuck to the fabric as a result of this oxidation process and making removal even more challenging.
Sensitivity to Temperature
High temperatures can cause blood stains to become more noticeable. Blood's proteins can become hydrolyzed and more strongly attach to cloth when heated, further establishing the stain.
Therefore, it may be more difficult to successfully remove blood stains when using hot water or heat during the washing process.
Penetration and Absorption
Particularly if the cloth is porous or absorbent in nature, blood can be quickly absorbed into its fibers. Blood can enter deeper into fabric fibers the longer it sits on the surface, which makes it harder to totally remove.
The proteins found in blood vibrate until the connections holding it together are broken by hot temperatures which causes the protein to clump. You should always use cold water to remove blood stains from clothing because once they are attached, water loses its power to wash them away.